منابع مشابه
Oocyte Meiotic Spindle Assembly and Function.
Gametogenesis in animal oocytes reduces the diploid genome content of germline precursors to a haploid state in gametes by discarding ¾ of the duplicated chromosomes through a sequence of two meiotic cell divisions called meiosis I and II. The assembly of the microtubule-based spindle structure that mediates this reduction in genome content remains poorly understood compared to our knowledge of...
متن کاملHDAC8 functions in spindle assembly during mouse oocyte meiosis
HDAC8 is a class I histone deacetylase that functions in a variety of biological processes through its non-histone substrates. However, its roles during oocyte meiosis remain elusive. Here, we document that HDAC8 localizes at spindle poles and positively participates in the regulation of microtubule organization and spindle assembly in mouse oocytes. Depletion of HDAC8 by siRNA-based gene silen...
متن کاملCorrection: evidence for the requirement of 14-3-3eta (YWHAH) in meiotic spindle assembly during mouse oocyte maturation
Correction After publication of this work [1] we determined that the one of the experiments used an incorrect combination of primary and secondary antibodies. In the Results and Discussion and in the Methods we referred to an experiment which used a goat anti-14-3-3η antibody. In combination with this antibody, a secondary antibody used in the experiment was incorrect. It would, in fact be labe...
متن کاملEffect of dehydroepiandrosterone on meiotic spindle structure and oocyte quality in mice
Objective(s): Dehydroepiandrosterone (DHEA) has been reported to improve pregnancy chances in women with diminished ovarian reserve (DOR) and to reduce miscarriage rates by 50–80%. This study, therefore, assesses effects of DHEA on number of retrieved oocytes and meiotic spindles.Materials and Methods: A randomized, prospective, controlled study was conducted on eight groups, four groups of you...
متن کاملEZH2 is required for mouse oocyte meiotic maturation by interacting with and stabilizing spindle assembly checkpoint protein BubRI
Enhancer of zeste homolog 2 (EZH2) trimethylates histone H3 Lys 27 and plays key roles in a variety of biological processes. Stability of spindle assembly checkpoint protein BubR1 is essential for mitosis in somatic cells and for meiosis in oocytes. However, the role of EZH2 in oocyte meiotic maturation was unknown. Here, we presented a mechanism underlying EZH2 control of BubR1 stability in th...
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ژورنال
عنوان ژورنال: Molecular Reproduction and Development
سال: 2013
ISSN: 1040-452X
DOI: 10.1002/mrd.22275